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產(chǎn)品分類技術(shù)文章/ Technical Articles
Assay Biotechnology 重組蛋白和合成肽
Assay Biotechnolog公司自2006年1月成立以來(lái),Assay Biotechnology一直是業(yè)界ling xian 的抗體生產(chǎn)和工程和測(cè)定技術(shù),熒光染料,猝滅劑,重組蛋白和合成肽的全球貢獻(xiàn)者。位于加利福尼亞州舊金山灣區(qū)的心臟地帶。
在我們成立后的頭幾年,Assay Biotech成立了抗體制造商和抗體工程公司,通過(guò)OEM合作伙伴關(guān)系專門針對(duì)大型公司。從那時(shí)起,我們一直在成為一個(gè)突出的測(cè)定和抗體來(lái)源,將其覆蓋范圍擴(kuò)大到全球超過(guò)50個(gè)國(guó)家的數(shù)百萬(wàn)研究人員。
我們一直在尋求推動(dòng)我們潛力的信條,決心成為業(yè)界的測(cè)定開(kāi)發(fā)和制造公司之一,提供大量即用型測(cè)定,免疫試劑,分子檢測(cè)模塊,生物標(biāo)志物和全線抗體工程服務(wù)支持zui艱巨的學(xué)術(shù),藥學(xué)和臨床研究工作。
協(xié)議:
將所有試劑制備至工作濃度,將標(biāo)準(zhǔn)品制備至所需范圍,并將樣品制備至適當(dāng)?shù)南♂屢蜃印?/span>
取出所需數(shù)量的捕獲抗體包被條進(jìn)行實(shí)驗(yàn),并將剩余的條帶放回裝有干燥劑的干燥袋中,進(jìn)行 4°C 儲(chǔ)存。
向每個(gè)孔中加入100μl標(biāo)準(zhǔn)品/樣品,并在室溫(RT)下在軌道振蕩器上孵育2小時(shí)。
吸出溶液,向每個(gè)正在使用的孔中加入300μl1x洗滌緩沖液,并在軌道振蕩器上輕輕振蕩2-3分鐘。重復(fù)此過(guò)程 3 次。最后一次洗滌后,通過(guò)倒置盤子并將其敲擊在干凈的紙巾上,確保沒(méi)有液體殘留。
向每個(gè)孔中加入 100 μl 1x 檢測(cè)抗體并孵育 2 小時(shí)。在 RT 的軌道振動(dòng)臺(tái)上
重復(fù)步驟 4。
向每個(gè)孔中加入100μl1x SAV-HRP,并在室溫軌道振蕩器上孵育30分鐘
重復(fù)步驟 4。
向每個(gè)孔中加入100μl復(fù)合增強(qiáng)劑/過(guò)氧化物溶液,蓋板免受光照,并在室溫軌道振蕩器上孵育5分鐘。
在光度計(jì)上讀取板。
基于比色的細(xì)胞ELISA
基于比色的基于細(xì)胞的 ELISA 試劑盒可檢測(cè)各種靶蛋白以及某些刺激條件對(duì)不同細(xì)胞系中靶蛋白表達(dá)的影響。通過(guò)間接ELISA形式實(shí)現(xiàn)目標(biāo)蛋白濃度的定性測(cè)定。
從本質(zhì)上講,靶蛋白被靶標(biāo)特異性一抗 (1°) 捕獲,而 HRP 偶聯(lián)的二抗 (2°) 結(jié)合 1° 抗體的 Fc 區(qū)域。通過(guò)這種結(jié)合,與 2° 抗體偶聯(lián)的 HRP 酶可以在添加底物時(shí)催化比色反應(yīng)。
由于基于細(xì)胞的 ELISA 的定性性質(zhì),描述了多種歸一化方法:1) 包括對(duì)人 GAPDH 具有特異性的單克隆抗體,作為歸一化目標(biāo)吸光度值的內(nèi)部陽(yáng)性對(duì)照。2)通過(guò)添加底物對(duì)HRP活性進(jìn)行比色測(cè)量后,采用結(jié)晶紫全細(xì)胞染色法測(cè)定細(xì)胞密度。
染色后,可以通過(guò)將吸光度值歸一化為細(xì)胞量來(lái)分析結(jié)果,從而可以調(diào)整鍍層差異。3) 如果檢測(cè)到磷酸化靶標(biāo),將提供針對(duì)非磷酸化對(duì)應(yīng)物的抗體用于標(biāo)準(zhǔn)化目的。獲得的非磷酸化靶標(biāo)的吸光度值可用于歸一化磷酸化靶標(biāo)的吸光度值。
下面是Assay Biotechnology公司的部分熱銷產(chǎn)品
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
Tryptophan Hydroxylase (Phospho-Ser260) Antibody | P12-1295-100 | 100 ug |
HSL (Phospho-Ser855/554) Antibody | A0074-50 | 50 ug |
PFKFB2 (Phospho-Ser483) Antibody | A0761-50 | 50 ug |
eNOS (Phospho-Ser615) Antibody | A1145-50 | 50 ug |
PYK2 (Phospho-Tyr402) Antibody | A7204-50 | 50 ug |
SREBP-1 (Phospho-Ser439) Antibody | A8425-50 | 50 ug |
SP1 Antibody | B0577-50 | 50 ug |
Tryptophan Hydroxylase Antibody | B1011-50 | 50 ug |
IRS-1 Antibody | B7120-50 | 50 ug |
Paxillin Antibody | B8250-50 | 50 ug |
OCT2 Antibody | C0282-50 | 50 ug |
HARS Antibody | C10349-50 | 50 ug |
CGK 2 Antibody | C10961-50 | 50 ug |
IRX2 Antibody | C11651-50 | 50 ug |
Collagen IX alpha3 Antibody | C12212-50 | 50 ug |
ABHD12 Antibody | C14209-50 | 50 ug |
ST6GAL1 Antibody | C15170-50 | 50 ug |
AASDHPPT Antibody | C16447-50 | 50 ug |
APOBEC3D/F Antibody | C17872-50 | 50 ug |
SFRS15 Antibody | C18932-50 | 50 ug |
THY1 Antibody | C21278-50 | 50 ug |
ITGAD Antibody | C30328-50 | 50 ug |
SAMHD1 Antibody | C30986-50 | 50 ug |
GNRHR Antibody | G276-50 | 50 ug |
OR6K2 Antibody | G663-50 | 50 ug |
Caspase 6 (Cleaved-Asp162) Antibody | L0106-50 | 50 ug |
S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody | P12-1174-50 | 50 ug |
Lyn Antibody | R12-2230-50 | 50 ug |
ANGPTL7 Antibody | R12-2486-50 | 50 ug |
EIF4E3 Antibody | R12-2742-50 | 50 ug |
MARK3 Antibody | R12-2998-50 | 50 ug |
OR6P1 Antibody | R12-3254-50 | 50 ug |
SIRT3 Antibody | R12-3510-50 | 50 ug |
eEF2K (Phospho-Ser366) Antibody | A0071-100 | 100 ug |
14-3-3 thet/tau (Phospho-Ser232) Antibody | A0758-100 | 100 ug |
Catenin-alpha1 (Phospho-Ser641) Antibody | A1140-100 | 100 ug |
PTEN (Phospho-Ser370) Antibody | A7201-100 | 100 ug |
Gab2 (Phospho-Ser623) Antibody | A8350-100 | 100 ug |
p70 S6 Kinase Antibody | B0534-100 | 100 ug |
HER3 Antibody | B0946-100 | 100 ug |
FKHRL1 Antibody | B7087-100 | 100 ug |
RSK1/2/3/4 Antibody | B8182-100 | 100 ug |
Keratin 10 Antibody | C0249-100 | 100 ug |
MOS Antibody | C10258-100 | 100 ug |
Mevalonate Kinase Antibody | C10868-100 | 100 ug |
TREF1 Antibody | C11526-100 | 100 ug |
CDC5L Antibody | C12171-100 | 100 ug |
PRKAG1 Antibody | C14134-100 | 100 ug |
CBLN2 Antibody | C15057-100 | 100 ug |
GBP3 Antibody | C16080-100 | 100 ug |
4E-BP1 Monoclonal Antibody | N0001 | 50ul |
A1BG Monoclonal Antibody | N0004 | 50ul |
AATM Monoclonal Antibody | N0006 | 50ul |
AIF-M1 Monoclonal Antibody | N0017 | 50ul |
YB1 (Phospho-Ser102) Antibody | P12-1200 | 50ul |
Tryptophan Hydroxylase (Phospho-Ser260) Antibody | P12-1195 | 50ul |
TIF-IA (Phospho-Ser649) Antibody | P12-1189 | 50ul |
STMN1 (Phospho-Ser62) Antibody | P12-1182 | 50ul |
MAP2K7 (Phospho-Thr275) Antibody | P12-1140 | 50ul |
LIMK2 (Phospho-Thr505) Antibody | P12-1137 | 50ul |
GRK2 (Phospho-Ser685) Antibody | P12-1112 | 50ul |
FLT3 (Phospho-Tyr969) Antibody | P12-1108 | 50ul |
FAK (Phospho-Tyr397) Antibody | P12-1103 | 50ul |
B-RAF (Phospho-Ser446) Antibody | P12-1080 | 50ul |
ATF1 (Phospho-Ser63) Antibody | P12-1077 | 50ul |
AhR (Phospho-Ser36) Antibody | P12-1072 | 50ul |
ATP-citrate synthase (Phospho-Ser455) Antibody | P12-1003 | 50ul |
ACK (Phospho-Tyr518) Antibody | P12-1001 | 50ul |
EGFR (Phospho-Thr693) Antibody | A0009 | 50ul |
HSP90β (Phospho-Ser254) Antibody | A0013 | 50ul |
ALK (Phospho-Tyr1586) Antibody | P12-1002 | 50ul |
Adducin α/β (Phospho-Ser726/713) Antibody | A0002 | 50ul |
GSK3α/β (Phospho-Tyr279/216) Antibody | A0012 | 50ul |
C/EBP α (Phospho-Ser21) Antibody | A0056 | 50ul |
Caspase-9 (Phospho-Thr125) Antibody | A0060 | 50ul |
eEF2K (Phospho-Ser366) Antibody | A0071 | 50ul |
Cyclin D1 (Phospho-Thr286) Antibody | A0417 | 50ul |
Goat Anti-Chicken IgY (H&L) - Cy7 | SA1257 | 50ul |
Goat Anti-Human IgG gamma chain - Cy7 | SA1261 | 50ul |
Goat Anti-Human IgM mu chain - Cy7 | SA1263 | 100 ul |
Goat Anti-Rabbit IgG (H&L) - Cy7 | SA1267 | 100 ul |
Rabbit Anti-Chicken IgY (H&L) - Cy7 | SA1270 | 100 ul |
Rabbit Anti-Guinea Pig IgG (H&L) - Cy7 | SA1275 | 100 ul |
Mouse Anti-Human Ig lambda chain - Cy7 | SA1297 | 100 ul |
Rabbit Anti-Human IgG F(ab')2 - Cy7 | SA1299 | 100 ul |
Rabbit Anti-Human IgG (H&L) - Cy7 | SA1277 | 100 ul |
Rabbit Anti-Human IgG gamma chain - Cy7 | SA1279 | 100 ul |
Rabbit Anti-Monkey IgG (H&L) - Cy7 | SA1282 | 100 ul |
Goat Anti-Chicken IgY (H&L) - AMCA | SA0440 | 100 ul |
Goat Anti-Human IgG Fab - AMCA | SA0442 | 100 ul |
Goat Anti-Human IgG+IgM+IgA - AMCA | SA0445 | 100 ul |
Goat Anti-Rabbit IgG (H&L) - AMCA | SA0450 | 100 ul |
Goat Anti Chicken IgY (H&L) - Alexa Fluor 750 | SA1042 | 100 ul |
Goat Anti Rabbit IgG (H&L) - Alexa Fluor 750 | SA1052 | 100 ul |
Rabbit Anti Dog IgG (H&L) - Alexa Fluor 750 | SA1056 | 100 ul |
Rabbit Anti Guinea Pig IgG (H&L) - Alexa Fluor 750 | SA1060 | 100 ul |
Rabbit Anti Human IgG Fc - Alexa Fluor 750 | SA1063 | 100 ul |
Rabbit Anti Human IgG+IgM+IgA - Alexa Fluor 750 | SA1065 | 100 ul |
Rabbit Anti Monkey IgG (H&L) - Alexa Fluor 750 | SA1067 | 100 ul |
Rabbit Anti Sheep IgG (H&L) - Alexa Fluor 750 | SA1071 | 100 ul |
Mouse Anti Human IgG Fab - Alexa Fluor 750 | SA1075 | 100 ul |
Mouse Anti Human IgM - Alexa Fluor 750 | SA1077 | 100 ul |
Mouse Anti Human Ig kappa chain - Alexa Fluor 750 | SA1081 | 100 ul |
BMAL1 (Acetyl-Lys538) Colorimetric Cell-Based ELISA Kit | CBA1086 | 1 Kit |
p73 (Acetyl-Lys327) Colorimetric Cell-Based ELISA Kit | CBA1085 | 1 Kit |
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